A phosphate buffer solution is a handy buffer to have around, especially for biological applications. Since the osmolarity matches that of the human body, PBS is frequently used during cell model washing preparations whilst preventing cell lysis. The three pKa values for phosphoric acid (from the CRC … This wide range is due to phosphoric acid having 3 dissociation constants, (known in chemistry as a triproti Download the recipe as a PDF Phosphate Buffer (pH 5.8 to 7.4) preparation guide and recipe. The goal of a buffer solution is to help maintain a stable pH when a small amount of acid or base is introduced into a solution. Recipe can be automatically scaled by entering desired final volume. A phosphate buffer solution is especially useful for biological applications, which are especially sensitive to pH changes since it is possible to prepare a solution near any of three pH levels. A simple phosphate buffer is used ubiquitously in biological experiments, as it can be adapted to a variety of pH levels, including isotonic. Because phosphoric acid has multiple dissociation constants, you can prepare phosphate buffers near any of the three pHs, which are at 2.15, 6.86, and 12.32. Phosphate buffered saline (PBS) solution is a commonly used buffer used in a variety of biological applications in order to maintain the pH of a solution.

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